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HERO ID
2238180
Reference Type
Journal Article
Title
Protein tyrosine nitration and thiol oxidation by peroxynitrite-strategies to prevent these oxidative modifications
Author(s)
Daiber, A; Daub, S; Bachschmid, M; Schildknecht, S; Oelze, M; Steven, S; Schmidt, P; Megner, A; Wada, M; Tanabe, T; Münzel, T; Bottari, S; Ullrich, V
Year
2013
Is Peer Reviewed?
Yes
Journal
International Journal of Molecular Sciences
ISSN:
1422-0067
EISSN:
14220067
Volume
14
Issue
4
Page Numbers
7542-7570
Language
English
PMID
23567270
DOI
10.3390/ijms14047542
Web of Science Id
WOS:000318017100051
Abstract
The reaction product of nitric oxide and superoxide, peroxynitrite, is a potent biological oxidant. The most important oxidative protein modifications described for peroxynitrite are cysteine-thiol oxidation and tyrosine nitration. We have previously demonstrated that intrinsic heme-thiolate (P450)-dependent enzymatic catalysis increases the nitration of tyrosine 430 in prostacyclin synthase and results in loss of activity which contributes to endothelial dysfunction. We here report the sensitive peroxynitrite-dependent nitration of an over-expressed and partially purified human prostacyclin synthase (3.3 μM) with an EC50 value of 5 μM. Microsomal thiols in these preparations effectively compete for peroxynitrite and block the nitration of other proteins up to 50 μM peroxynitrite. Purified, recombinant PGIS showed a half-maximal nitration by 10 μM 3-morpholino sydnonimine (Sin-1) which increased in the presence of bicarbonate, and was only marginally induced by freely diffusing NO2-radicals generated by a peroxidase/nitrite/hydrogen peroxide system. Based on these observations, we would like to emphasize that prostacyclin synthase is among the most efficiently and sensitively nitrated proteins investigated by us so far. In the second part of the study, we identified two classes of peroxynitrite scavengers, blocking either peroxynitrite anion-mediated thiol oxidations or phenol/tyrosine nitrations by free radical mechanisms. Dithiopurines and dithiopyrimidines were highly effective in inhibiting both reaction types which could make this class of compounds interesting therapeutic tools. In the present work, we highlighted the impact of experimental conditions on the outcome of peroxynitrite-mediated nitrations. The limitations identified in this work need to be considered in the assessment of experimental data involving peroxynitrite.
Keywords
nitric oxide; superoxide; peroxynitrite; protein tyrosine nitration; thiol oxidation; peroxynitrite scavengers; prostacyclin synthase
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Nitrate/Nitrite
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