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2264183 
Journal Article 
Abstract 
Nitric oxide plays a different role on DCs as anti-inflammatory, pro inflammatory or induces Th17 depending on the concentration and time point used 
Graf, L; Cesson, V; Nicod, L; Obregon, C 
2010 
Yes 
American Journal of Respiratory and Critical Care Medicine
ISSN: 1073-449X
EISSN: 1535-4970 
181 
A1269 
English 
WOS:000208771000270 
is part of a larger document 3452678 Proceedings of the American Thoracic Society 2010 International Conference, May 14-19, 2010, New Orleans
In the lung dendritic cells (DC), are underneath epithelial barrier and continuously influenced by the antigens and redox state. We are interested in the role that reactive oxygen species such as nitric oxide (NO) play in DC maturation. Human DCs were simulated with a long acting NO donor before and after LPS maturation. Dose responses and time dependent experiments were achieved. DC-maturation markers, a panel Th1, Th2 and Th17 cytokines, intracellular phosphorylation of nuclear factors and proliferation assays were analyzed. Our results have shown that NO is able to change the pattern of the release of cytokines of LPS-matured DC which was dependent on the concentration of NO used, as well as in the time point that NO was added to the cells during the maturation. IL-10 and TNF-α were released in a dose dependent manner while the release of IL-12 was inhibited. If NO is added before maturation by LPS an important inhibition of IL-12 and IL-10 was observed. In contrast, if NO is added after maturation, it strengthen inflammatory response of mature DCs increasing the release of TNF-α and IL-12. Intracellularly, NO seem to modulate the phosphorylation state of ERK, when added before LPS-maturation. Functionally, in spite of the fact that co-stimulation of DC with NO and LPS did not modify allopresentation, NO treated DCs before maturation induced the release of IL-6 and Th17 cells. Low concentration of NO seem to have anti-inflammatory effect during maturation of DC. However an enhanced oxidizing environment after DC maturation, amplify the inflammatory response. Interestingly, NO might play an important role in autoimmunity inducing Th17. 
American Thoracic Society 2010 International Conference 
New Orleans, LA 
May 14-19, 2010