Bonding of cholesterol oxides to functionalised multiwalled carbon nanotubes for in-vitro biosensor application | Health & Environmental Research Online (HERO)

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Citation
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HERO ID

2277262

Reference Type

Journal Article

Title

Bonding of cholesterol oxides to functionalised multiwalled carbon nanotubes for in-vitro biosensor application

Author(s)

Malarvizhi, A; Pandurangan, A

Year

2012

Is Peer Reviewed?

Journal

Biomedical Research
ISSN: 0970-938X
EISSN: 0976-1683

Volume

Issue

Page Numbers

505-512

Web of Science Id

WOS:000318217500008

Abstract

MWCNTs (multiwalled carbon nanotubes) were synthesized by chemical vapour deposition (CVD) and functionalised using Hcl and HNO3 at 80 degrees C. Their FTIR spectra revealed the characteristic peaks for carboxylic acid and alcoholic groups. Both unfunctionalized (MWCNTs) and functionalised multiwalled carbon nanotubes (f-MWCNTs) were reacted with cholesterol oxidase using 1-ethyl-3-(3-dimethylaminopropyl) carbodimide and N-hydroxyl sulfo-succinimide. But bonding with cholesterol oxidase was established only with f-MWCNTs. Cholesterol oxidase bonded to f-MWCNTs was tested for cholesterol sensing using cyclic voltametry. It established interaction of cholesterol with cholesterol oxidase bonded to f-MWCNTs. Hence cholesterol oxidase bonded to f-MWCNTs could be a novel in-vitro cholesterol sensor in hyper lipidemia.

Keywords

Carbon nanotubes; Functionalized CNT; Cholesterol; Cholesterol oxidase; Cyclic voltammogram