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2279342 
Journal Article 
Determination of Organophosphorus and Carbamic Pesticides with an Acetylcholinesterase Amperometric Biosensor using 4-Aminophenyl Acetate as Substrate 
La Rosa, C; Pariente, F; Hernandez, L; Lorenzo, E 
1994 
Yes 
Analytica Chimica Acta
ISSN: 0003-2670
EISSN: 1873-4324 
ELSEVIER SCIENCE BV 
AMSTERDAM 
NIOSH/00223930 
295 
3 
273-282 
English 
WOS:A1994PH14600006 
A proposed technique for determining organophosphate and carbamate pesticides using an acetylcholinesterase (AChE) amperometric biosensor was developed. The sensor consisted of electric-eel AChE immobilized on the surface of a glassy carbon electrode (GCE) through a glutaraldehyde crosslinked layer which was inserted into a solution of 1.2 millimolar (mM) 4-aminophenyl-acetate (PAPA), which served as the substrate. The AChE hydrolyzed the PAPA to 4-aminophenol (PAP) which then underwent oxidation by the GCE, which was biased at 250 millivolts relative to a sodium-chloride saturated calomel electrode. In the presence of an organophosphate or carbamate pesticide, AChE in the sensor was inhibited which led to a decrease in the amount of PAPA that was hydrolyzed to PAP. The decrease in PAP formation, and consequently the degree of AChE inhibition, could be followed by cyclic voltammetry since the peak current was proportional to the bulk concentration of an electroactive species. This enabled correlation with the concentration of pesticide present in the solution. The technique was applied to determining paraoxon (311455) and carbaryl (63252) in a series of standard solutions. After 3 minutes preincubation in a phosphate buffer, pH 7.9, paraoxon and carbaryl could be detected at concentrations as low as 4.0 and 13.0 nanomoles per liter (nmol/l), respectively. Five replicate determinations of 1.0 micromolar paraoxon yielded a relative standard deviation of less than 5%. When applied to analyzing spiked samples of natural spring water, 9.5nmol/l carbaryl and 8.8nmol/l paraoxon could be detected. 
Amperometry; Biosensors; Enzymatic methods; Pesticides; acetylcholinesterase; benomyl; carbamate pesticide; carbaril; carbofuran; organophosphate pesticide; paraoxon; parathion; amperometry; article; biosensor; priority journal; quantitative assay; water analysis; water pollution 
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