Health & Environmental Research Online (HERO)


Print Feedback Export to File
2286551 
Journal Article 
The in-vitro antibacterial effects of organic salts, chemical disinfectants and antibiotics against pathogens of black disease in fairy shrimp of Thailand 
Saejung, C; Hatai, K; Sanoamuang, L 
2014 
Yes 
Journal of Fish Diseases
ISSN: 0140-7775
EISSN: 1365-2761 
37 
1 
33-41 
24131294 
WOS:000328421100005 
The antibacterial effects of organic salts, chemical disinfectants and antibiotics were evaluated on cultures of Aeromonas hydrophila C03, Aeromonas sobria C26, A.sobria C29, Aeromonascaviae C24 and Acinetobacter sp. SH-94B, the pathogens that cause black disease found in fairy shrimps (Streptocephalus sirindhornae Sanoamuang et al. (2000) and Branchinella thailandensis Sanoamuang, Saengphan & Murugan) of Thailand. The minimal inhibitory concentrations (MICs) of organic salts (sodium chloride and potassium chloride) and antibiotics (oxytetracycline dihydrate, streptomycin sulphate, kanamycin monosulphate, chloramphenicol and ampicillin) were determined using the agar-dilution method. The effect of chemical disinfectants (sodium hypochlorite and chlorine dioxide) was evaluated by exposing bacteria to different concentrations of these chemicals for different periods of time. Interestingly, all strains were intrinsically resistant to 0.25-3% sodium chloride and potassium chloride. The effect of sodium hypochlorite was greater than that of chlorine dioxide, and 5-20gmL(-1) of sodium hypochlorite was sufficient to inhibit the growth of these bacteria, but the exposure time varied, depending on the bacterial species. Of the antibiotics tested, chloramphenicol and oxytetracycline dihydrate completely inhibited the selected strains. Chloramphenicol showed the highest antibacterial effect against all pathogenic species - the MIC and minimal bactericidal concentration (MBC) ranged from 0.8 to 3.1gmL(-1) from 3.1 to 6.25gmL(-1), respectively. To achieve control of black disease during cultivation of fairy shrimp, data derived from this study can be used as a basis for further toxicity tests in vivo. 
Acinetobacter sp; Aeromonas spp; black spot; Branchinella thailandensis; fairy shrimp; Streptocephalus sirindhornae