Health & Environmental Research Online (HERO)


Print Feedback Export to File
2292369 
Journal Article 
Comparative proteomic analysis of cysteine oxidation in colorectal cancer patients 
Yang, HeeY; Chay, KeeOh; Kwon, J; Kwon, SOh; Park, YKyu; Lee, TaeH 
2013 
1 
Molecules and Cells
ISSN: 1016-8478
EISSN: 0219-1032 
35 
6 
533-542 
23677378 
WOS:000321131500009 
Oxidative stress promotes damage to cellular proteins, lipids, membranes and DNA, and plays a key role in the development of cancer. Reactive oxygen species disrupt redox homeostasis and promote tumor formation by initiating aberrant activation of signaling pathways that lead to tumorigenesis. We used shotgun proteomics to identify proteins containing oxidation-sensitive cysteines in tissue specimens from colorectal cancer patients. We then compared the patterns of cysteine oxidation in the membrane fractions between the tumor and non-tumor tissues. Using nano-UPLC-MSE proteomics, we identified 31 proteins containing 37 oxidation-sensitive cysteines. These proteins were observed with IAM-binding cysteines in non-tumoral region more than tumoral region of CRC patients. Then using the Ingenuity pathway program, we evaluated the cellular canonical networks connecting those proteins. Within the networks, proteins with multiple connections were related with organ morphology, cellular metabolism, and various disorders. We have thus identified networks of proteins whose redox status is altered by oxidative stress, perhaps leading to changes in cellular functionality that promotes tumorigenesis. 
colorectal cancer; cysteine oxidation; iodoacetamide; protein network; shotgun proteomics