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2328395 
Journal Article 
In vitro induction and enlargement of apical domes and formation of multiple shoots in finger millet, Eleusine coracana (L.) Gaertn and crowfoot grass, Eleusine indica (L.) Gaertn 
Kumar, S; Agarwal, K; Kothari, SL 
2001 
Yes 
Current Science
ISSN: 0011-3891
EISSN: 0011-3905 
INDIAN ACAD SCIENCES 
BANGALORE 
81 
11 
1482-1485 
English 
WOS:000172794500034 
In cereal crops, somatic embryogenesis is the most common pathway of plant regeneration. We describe a protocol for high frequency regeneration of Eleusine coracana via formation of large apical domes from seeds, mature embryos, immature inflorescences and immature embryos. The induction of apical domes occurred on MS medium supplemented with different auxins [2,4-dichlorophenoxyacetic acid (2,4-D); 2,4,5-trichlorophenoxyacetic acid (2,4,5-T); and parachlorophenoxyacetic acid (pCPA)] and cytokinins [kinetin (Kn) and 6-benzylaminopurine (BAP)]. The primary domes, after four weeks of subculturing on MS + 2,4-D (0.1, 0.2 mg/l), proliferated rapidly and gave rise to secondary and tertiary domes along with green, nodular, compact callus. The domes on MS medium containing GA3; (1 mg/l) or 1-naphthalene-acetic acid (NAA) (1 mg/l) gave rise to high-frequency shoot bud differentiation. Multiple shoot buds arose over the entire surface of the dome, exogenously. Histological observations provided a clear evidence that the shoot apices of the mature and immature embryos transformed into enlarged apical domes and the surface of the domes was occupied by highly meristematic cells which differentiated into shoot buds. De novo differentiation of meristematic giant domes and shoot buds was also observed in the repeatedly subcultured callus raised from mature embryos as well as inflorescence. Similar structures were also seen in cultures raised from immature inflorescence of crowfoot grass (Eleusine indica).