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Citation
Tags
HERO ID
2332315
Reference Type
Journal Article
Title
Proinflammatory effects and oxidative stress within human bronchial epithelial cells exposed to atmospheric particulate matter (PM2.5 and PM > 2.5) collected from Cotonou, Benin
Author(s)
Cachon, BF; Firmin, S; Verdin, A; Ayi-Fanou, L; Billet, S; Cazier, F; Martin, PJ; Aissi, F; Courcot, D; Sanni, A; Shirali, P
Year
2014
Is Peer Reviewed?
Yes
Journal
Environmental Pollution
ISSN:
0269-7491
EISSN:
1873-6424
Volume
185
Page Numbers
340-351
Language
English
PMID
24333687
DOI
10.1016/j.envpol.2013.10.026
Web of Science Id
WOS:000331672500041
Abstract
After particulate matter (PM) collection in Cotonou (Benin), a complete physicochemical characterization of PM2.5 and PM>2.5 was led. Then, their adverse health effects were evaluated by using in vitro culture of human lung cells. BEAS-2B (bronchial epithelial cells) were intoxicated during short-term exposure at increasing PM concentrations (1.5-96 μg/cm(2)) to determine global cytotoxicity. Hence, cells were exposed to 3 and 12 μg/cm(2) to investigate the potential biological imbalance generated by PM toxicity. Our findings showed the ability of both PM to induce oxidative stress and to cause inflammatory cytokines/chemokines gene expression and secretion. Furthermore, PM were able to induce gene expression of enzymes involved in the xenobiotic metabolism pathway. Strong correlations between gene expression of metabolizing enzymes, proinflammatory responses and cell cycle alteration were found, as well as between proinflammatory responses and cell viability. Stress oxidant parameters were highly correlated with expression and protein secretion of inflammatory mediators.
Keywords
Benin; Cytotoxicity; Inflammatory response; Oxidative stress; Particulate matter
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