US EPA

2453376 

Journal Article 

Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa 

Zhang, W; Chen, S; Liao, Y; Wang, D; Ding, J; Wang, Y; Ran, X; Lu, D; Zhu, H 

2013 

1 

Protein Expression and Purification
ISSN: 1046-5928 

92 

2 

208-213 

English 

24125754 

10.1016/j.pep.2013.09.017 

WOS:000327228800012 

http://dx.doi.org/10.1016/j.pep.2013.09.017
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As a member of zinc-containing medium-chain alcohol dehydrogenase family, formaldehyde dehydrogenase (FDH) can oxidize toxic formaldehyde to less active formate with NAD(+) as a cofactor and exists in both prokaryotes and eukaryotes. Most FDHs are well known to be glutathione-dependent in the catalysis of formaldehyde oxidation, but the enzyme from Pseudomonas putida is an exception, which is independent of glutathione. To identify novel glutathione-independent FDHs from other bacterial strains and facilitate the corresponding structural and enzymatic studies, high-level soluble expression and efficient purification of these enzymes need to be achieved. Here, we present molecular cloning, expression, and purification of the FDH from Pseudomonas aeruginosa, which is a Gram-negative pathogenic bacterium causing opportunistic human infection. The FDH of P. aeruginosa shows high sequence identity (87.97%) with that of P. putida. Our results indicated that coexpression with molecular chaperones GroES, GroEL, and Tig has significantly attenuated inclusion body formation and improved the solubility of the recombinant FDH in Escherichia coli cells. A purification protocol including three chromatographic steps was also established to isolate the recombinant FDH to homogeneity with a yield of similar to 3.2 mg from 1 L of cell culture. The recombinant P. aeruginosa FDH was properly folded and biologically functional, as demonstrated by the mass spectrometric, crystallographic, and enzymatic characterizations of the purified proteins. (C) 2013 Elsevier Inc. All rights reserved. 

Pseudomonas aeruginosa; Formaldehyde dehydrogenase; Molecular chaperone; Coexpression; Protein purification; Characterization