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Citation
Tags
HERO ID
2454828
Reference Type
Journal Article
Subtype
Review
Title
Histopathology for the diagnosis of infectious diseases
Author(s)
Gupta, E; Bhalla, P; Khurana, N; Singh, T
Year
2009
Is Peer Reviewed?
Yes
Journal
Indian Journal of Medical Microbiology
ISSN:
0255-0857
EISSN:
1998-3646
Publisher
Medknow Publications & Media Pvt. Ltd.
Volume
27
Issue
2
Page Numbers
100-106
Language
English
PMID
19384030
DOI
10.4103/0255-0857.49423
Web of Science Id
WOS:000267952400003
URL
http://dx.doi.org/10.4103/0255-0857.49423
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Abstract
Histopathological examination of tissue biopsies for the identification of infectious organisms is a very important diagnostic tool. Conventional culture confirmation of tissue biopsies often fail to identify any pathogen as, first of all, invariably most of the tissue samples that are collected and sent for culture isolation are inappropriately collected in formalin, which prevents pathogen growth in culture media. Inadequate processing like grinding, etc. further hinders isolation. Presence of inhibitors like dead tissue debris, fibers, etc. also delays isolation. Microbiologists often lack expertise in identifying infectious pathogens directly from tissue biopsies by microscopic visualization. This review therefore acquaints microbiologists with the various methods available for detecting infectious agents by using histological stains. On histopathological examination of the tissue biopsy once, it is determined that a disease is likely to be due to an infection and has characterized the inflammatory response and hence associated microorganisms should be thoroughly looked for. Although some microorganisms or their cytopathic effects may be clearly visible on routine haematoxylin- and eosin-stained sections, additional histochemical stains are often needed for their complete characterization. Highly specific molecular techniques, such as immunohistochemistry, in situ hybridization and nucleic acid amplification, may be needed in certain instances to establish the diagnosis of infection. Through appropriate morphologic diagnoses and interlaboratory communication and collaboration, direct microscopic visualization of tissue samples can thus be very helpful in reaching a correct and rapid diagnosis.
Keywords
Histopathology; infections; tissue biopsy
Tags
IRIS
•
Formaldehyde [archived]
Inflammation/Reactive Oxygen Species
WOS
PubMed
Screened by Title/Abstract
Not Primary Study: Review, Commentary, Abstract, Policy, Current Practice, Workplace Assessment
Retroactive RIS import
2014
HERO_Formaldehyde_InflammationReactiveOxygenSpecies_pid_31_uid_5713Sorting091214
HERO_Formaldehyde_InflammationReactiveOxygenSpecies_pid_31_uid_5713
Screened (Title/Abstract)
Not Primary Study: Review, Commentary, Abstract, Policy, Current Practice, Workplace Assessment, etc
•
IRIS Formaldehyde (Inhalation) [Final 2024]
Literature Indexing
PubMed
WoS
Literature Identification
Inflammation and Immune-Related Mechanistic Studies
Supplemental or not primary research
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