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HERO ID
2510769
Reference Type
Journal Article
Title
Antagonistic effects of a mixture of low-dose nonylphenol and di-n-butyl phthalate (monobutyl phthalate) on the Sertoli cells and serum reproductive hormones in prepubertal male rats in vitro and in vivo
Author(s)
Hu, Y; Wang, R; Xiang, Z; Qian, W; Han, X; Li, D
Year
2014
Is Peer Reviewed?
1
Journal
PLoS ONE
EISSN:
1932-6203
Book Title
PLoS One. 2014; 9(3):e93425. [PloS one]
Volume
9
Issue
3
Page Numbers
e93425
Language
English
PMID
24676355
DOI
10.1371/journal.pone.0093425
Web of Science Id
WOS:000333677500122
URL
https://search.proquest.com/docview/1510768141?accountid=171501
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Abstract
The estrogenic chemical nonylphenol (NP) and the antiandrogenic agent di-n-butyl phthalate (DBP) are regarded as widespread environmental endocrine disruptors (EDCs) which at high doses in some species of laboratory animals, such as mice and rats, have adverse effects on male reproduction and development. Given the ubiquitous coexistence of various classes of EDCs in the environment, their combined effects warrant clarification. In this study, we attempted to determine the mixture effects of NP and DBP on the testicular Sertoli cells and reproductive endocrine hormones in serum in male rats based on quantitative data analysis by a mathematical model. In the in vitro experiment, monobutyl phthalate (MBP), the active metabolite of DBP, was used instead of DBP. Sertoli cells were isolated from 9-day-old Sprague-Dawley rats followed by treatment with NP and MBP, singly or combined. Cell viability, apoptosis, necrosis, membrane integrity and inhibin-B concentration were tested. In the in vivo experiment, rats were gavaged on postnatal days 23-35 with a single or combined NP and DBP treatment. Serum reproductive hormone levels were recorded. Next, Bliss Independence model was employed to analyze the quantitative data obtained from the in vitro and in vivo investigation. Antagonism was identified as the mixture effects of NP and DBP (MBP). In this study, we demonstrate the potential of Bliss Independence model for the prediction of interactions between estrogenic and antiandrogenic agents.
Keywords
Sciences: Comprehensive Works; Sertoli cells; Apoptosis; Phthalates; Cell membranes; Cell viability testing; Hormones; Necrosis; Testosterone; Nonylphenol; Coexistence; Antagonism; Xenoestrogens; Food contamination & poisoning; n-Butyl phthalate; Laboratory animals; Life sciences; Immunology; Bisphenol A; Biology; Environmental impact; In vivo methods and tests; Rodents; Medical schools; Drug dosages; Butyl phthalate; Data processing; Data analysis; Viability; Endocrine disruptors; Analytical chemistry; Puberty; Medical research; Chemicals; Inhibin; Laboratory tests; Muridae
Tags
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Dibutyl Phthalate (DBP)
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Pubmed
Web of Science
Litsearch September 2014 - February 2015
WOS
LitSearch Jan 2014 - Sep 2014
PubMed
Web of Science
Studies with Supporting Data
Mechanistic and genotoxicity studies
Studies with Health Effects Data
Animal toxicology studies
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