In vivo formaldehyde cross-linking: it is time for black box analysis | Health & Environmental Research Online (HERO)

Health & Environmental Research Online (HERO)

Print Feedback Export to File

This record has one attached file:

Citation
Tags

HERO ID

2517714

Reference Type

Journal Article

Subtype

Letter

Title

In vivo formaldehyde cross-linking: it is time for black box analysis

Author(s)

Gavrilov, A; Razin, SV; Cavalli, G

Year

2014

Volume

Issue

Page Numbers

163-165

Language

English

PMID

25241225

DOI

10.1093/bfgp/elu037

Web of Science Id

WOS:000355693300007

Abstract

Formaldehyde cross-linking is an important component of many technologies, including chromatin immunoprecipitation and chromosome conformation capture. The procedure remains empirical and poorly characterized, however, despite a long history of its use in research. Little is known about the specificity of in vivo cross-linking, its efficiency and chemical adducts induced by the procedure. It is time to search this black box.

Keywords

formaldehyde cross-linking; chromatin; 3D genome organization; ChIP; 3C

Tags