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HERO ID
2549272
Reference Type
Journal Article
Title
Differential proinflammatory responses induced by diesel exhaust particles with contrasting PAH and metal content
Author(s)
Totlandsdal, AI; Låg, M; Lilleaas, E; Cassee, F; Schwarze, P
Year
2013
Is Peer Reviewed?
Yes
Journal
Environmental Toxicology
ISSN:
1520-4081
EISSN:
1522-7278
Volume
30
Issue
2
Page Numbers
188-196
Language
English
PMID
23900936
DOI
10.1002/tox.21884
Web of Science Id
WOS:000348661100007
Abstract
Exposure to diesel engine exhaust particles (DEPs), representing a complex and variable mixture of components, has been linked with cellular production and release of several types of mediators related to pulmonary inflammation. A key challenge is to identify the specific components, which may be responsible for these effects. The aim of this study was to compare the proinflammatory potential of two DEP-samples with contrasting contents of polycyclic aromatic hydrocarbons (PAHs) and metals. The DEP-samples were compared with respect to their ability to induce cytotoxicity, expression and release of proinflammatory mediators (IL-6, IL-8), activation of mitogen-activated protein kinases (MAPKs) and expression of CYP1A1 and heme oxygenase-1 (HO-1) in human bronchial epithelial (BEAS-2B) cells. In addition, dithiothreitol and ascorbic acid assays were performed in order to examine the oxidative potential of the PM samples. The DEP-sample with the highest PAH and lowest metal content was more potent with respect to cytotoxicity and expression and release of proinflammatory mediators, CYP1A1 and HO-1 expression and MAPK activation, than the DEP-sample with lower PAH and higher metal content. The DEP-sample with the highest PAH and lowest metal content also possessed a greater oxidative potential. The present results indicate that the content of organic components may be determinant for the proinflammatory effects of DEP. The findings underscore the importance of considering the chemical composition of particulate matter-emissions, when evaluating the potential health impact and implementation of air pollution regulations. © 2013 Wiley Periodicals, Inc. Environ Toxicol, 2013.
Keywords
diesel engine exhaust particles; PAH; metals; inflammation; epithelial lung cells
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