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2568381 
Journal Article 
An assay for measuring functional activated thrombin-activatable fibrinolysis inhibitor in plasma 
Kim, PYG; Foley, J; Hsu, G; Kim, PY; Neshelin, ME 
2008 
Yes 
Analytical Biochemistry
ISSN: 0003-2697
EISSN: 1096-0309 
372 
1 
32-40 
English 
17967438 
WOS:000251352900004 
Thrombin-activatable fibrinolysis inhibitor (TAFI), also called procarboxypeptidase U (proCPU), is a plasma zymogen that can be activated by thrombin, the thrombin-thrombomodulin complex, or plasmin. The activated form of TAFI (TAFIa, CPU) removes C-terminal lysine residues of plasmin-modified fibrin (FN') that mediates a positive feedback mechanism in plasminogen (Pg) activation, thereby attenuating fibrinolysis. The plasma concentration of TAR is approximately 75 nM. Because the half-maximal effect of TAFIa occurs at I nM, only approximately 1.3% of TAFI needs to be activated to exert an effect on clot lysis. The assay is performed by mixing soluble FN' covalently attached to a quencher and fluorescein-labeled Pg. The sample containing TAFIa is then added, and the rate of fluorescence increase due to removal of C-terminal lysine from FN' and loss of Pg binding is measured with a fluorescence plate reader. The assay was shown to be sensitive for TAFIa at a concentration as low as 12 pM. The intraassay variability and interassay variability of the assay were 6.3 and 8.3%, respectively. This assay was not confounded by the naturally occurring TAR Thr325Leu polymorphism that affects the thermal stability of TAFIa or endogenous plasminogen in plasma. (C) 2007 Elsevier Inc. All rights reserved. 
TAFIa; CPU; assay; fibrinolysis; plasma