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2596406 
Journal Article 
Desorption Electrospray Ionization Mass Spectrometry for Fast Detection of Alkaloids in Fructus Evodiae 
Chen Huan-Wen; Meng Jian; Wang Wei-Ping; Chen Chang-Lin; Wang Zhi-Chang 
2009 
Yes 
Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue
ISSN: 0253-3820 
37 
2 
237-241 
WOS:000265169000014 
Desorption electrospray ionization mass spectrometry(DESI-MS) was employed for fast direct detection of 5 alkaloids such as evodiamide, evodiamine, hydroxyvodiamine, rutae carpine and goshuyuamide-I presented in Fructus Evodiae herb plant. The operation parameters for both DESI and LTQ instrument were optimized and tested by previously examined plant tissue of Datura Stramonium so that a reliable fingerprint of the Fructus Evodiae tissue was generated successfully using a mixture of methanol/water/formic acid (49:49:2) as electrospray liquid. The alkaloids detected gave predominant signals at m/z 308, 304, 320, 288 and m/z 306, which were corresponding to the protonated molecules of evodiamide, evodiamine, hydroxyvodiamine, rutae carpine and goshuyuamide-I, respectively. The structures of the protonated molecules were confirmed with high confidence by tandem DESI-MS/MS, which was facilitated by the unique features of LTQ mass spectrometer. Ionic adducts of alkaloids and sodium were also commonly observed in DESI mass spectra of the plant tissue, and possible interference caused by dust, fake tissues were also investigated experimentally. A single run was completed in 1.5 min, providing a fast method for fingerprinting volatile compounds in plant tissue. It has been demonstrated that DESI is not only potentially useful for the quality monitoring of products in pharmaceutical processes, but also useful to discriminate authentic or fake Fructus Evodiae based on their mass spectral fingerprints. 
Desorption electrospray ionization; mass spectrometry; Fructus Evodiae; fast direct detection