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2605847 
Journal Article 
A survey of Penicillium brevicompactum and P-bialowiezense from indoor environments, with commentary on the taxonomy of the P-brevicompactum group 
Scott, JA; Wong, B; Summerbell, RC; Untereiner, WA 
2008 
Yes 
Botany
ISSN: 1916-2790
EISSN: 1916-2804 
86 
7 
732-741 
WOS:000257893400009 
We investigated the diversity of the Penicillium brevicompactum Dierckx group in dust from 54 houses in Wallaceburg, Ontario, Canada. Two taxa were predominant. P. brevicompactum and Pencillium bialowiezense Zaleski, accounting for 88.6% and 5.4% of the sample set, respectively. We further characterized multilocus haplotypes of isolates by characterizing three polymorphic genetic loci, (beta-tubulin (benA), historic 4 (his4A), and the internal transcribed spacer regions of ribosomal DNA (nucITS) amplified by PCR amplification and screened using heteroduplex mobility assay (HMA). Eight unique haplotypes were observed in P. brevicompactum s.str., and two in P. bialowiezense, both with a distribution characteristic of a predominantly clonal reproduction mode. Phylogenetic analysis of the (beta-tubulin and nucITS loci were carried out for members of the P. brevicompactum group, including ex-type material, that revealed three well-supported lineages corresponding to P. brevicompactum, P. bialowiezense (=Penicillium biourgeianum Zaleski), and Penicillium neocrassum R. Serra & S.W. Peterson. The mycophilic nature of many isolates of P. bialowiezense, and some isolates of P. brevicompactum, suggests that observation of members of the P. brevicompactum group in indoor environments may predict extensive and longterm fungal colonization. We also address some nomenclatural problems in the group and epitypify P. bialowiezense. 
DNA sequence analysis; dust biology; fungal population genetics; indoor air quality; indoor moulds; heteroduplex mobility assay