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2613063 
Journal Article 
Abstract 
Divergent airway inflammatory pathways of oxidative air pollutants 
Damewood, JB; Pourazar, J; Stenfors, N; Blomberg, A; Frew, A; Kelly, F; Sandstrom, T 
2010 
Yes 
American Journal of Respiratory and Critical Care Medicine
ISSN: 1073-449X
EISSN: 1535-4970 
181 
A1146 
English 
WOS:000208771000147 
is part of a larger document 3452678 Proceedings of the American Thoracic Society 2010 International Conference, May 14-19, 2010, New Orleans
Rationale: In spite of stricter regulations, ambient air pollution still remains a global health issue. Ozone (O3) and diesel exhaust (DE), both key oxidative air pollutants, have been demonstrated through numerous studies to pose hazardous health effects. Exposure to O3, as well as DE, has been shown to produce significant neutrophilia in the airways. It has previously been demonstrated that DE triggers an increase in nuclear translocation of NFκB and AP-1. The aim of the present study was to assess the expression of these transcription factors in the bronchial epithelium at baseline and after exposure to ozone in healthy subjects.

Methods: 15 healthy subjects underwent two separate 2-hour exposures, to filtered air as well as 0.2 ppm of O3. Bronchoscopy with endobronchial mucosal biopsy sampling was completed 6 hours post-exposure. Biopsies were processed in glycolmethacrylate (GMA) resin and immunostained for transcription factors.

Results: No significant changes were found in the bronchial epithelial expression of NFκB, C-fos or p-c-Jun post-ozone exposure as compared to air. This was true for total stainings as well as nuclear staining.

Conclusion: The activation of redox sensitive transcription factors after DE exposure could denote an underlying oxidative stress. However, in the current study, no analogous upregulation of these transcription factors was observed after an O3 challenge. These new data, taken in conjunction with previous results, further suggests that the inflammatory pathway triggered in the airways by ozone differs from that of diesel exhaust. 
American Thoracic Society 2010 International Conference 
New Orleans, LA 
May 14-19, 2010