Health & Environmental Research Online (HERO)


Print Feedback Export to File
2613179 
Journal Article 
Abstract 
Airways eosiniphilic inflammation in the airways of asthmatic children is correlated to particulate matter in induced sputum 
Fireman, E; Toledano, B; Soferman, R; Moshe, S; Sivan, Y; Kivity, S; Kokturk, N 
2010 
Yes 
American Journal of Respiratory and Critical Care Medicine
ISSN: 1073-449X
EISSN: 1535-4970 
181 
A1156 
English 
WOS:000208771000157 
is part of a larger document 3452678 Proceedings of the American Thoracic Society 2010 International Conference, May 14-19, 2010, New Orleans
Background: Air-borne particles may be responsible for the high prevalence of asthma, especially in children.

Population: 64 symptomatic children (aged 12.5±3 yrs) referred for induced sputum (IS) for differential diagnosis.

Methods: The children underwent pulmonary function tests by conventional methods, fractional nitric oxide testing by FeNO Ecomedics AG analyzer (Durnten, Switzerland), and IS by 20' ultrasonic nebulizer inhalation of 3% saline. Three-hundred cells were differentially counted in cytospin Giemsa‑stained slides. Analysis of IS particle size and shape was done by Eyetech Analyzers (Yokneam, Israel). TNF-α was measured in the IS supernatant by an ELISA kit (R&D SYSTEMS).

Results: The children were grouped into those with >3% eosinophils (Group I, n=39, age 12.4±3.37 yrs) or with <3% eosinophils (Group II, n=25, age 12.57±2.39 yrs). FEV1/FVC ratio and FeNO differed significantly between groups: 88.28±13.44% and 98.88±13.09 % (p<0.003), and 55.1±37.09 ppm and 15.92±9.39 ppm (p<0.000) in Group I and Group II, respectively. The difference in particulate matter size distribution (prtc <5 um) was significant between the groups: 4.26±1.56% in Group I and 3.36 ±2.0% in Group II (p<0.05). Eosinophils significantly (+) correlated to prtc size (3-4 µm, 4-5µm, 5-10µm) (r= 0.276, p<0.028, r= 0.385 p<0.002, r= 0.412, p<0.001 respectively). Rougher prtc were seen in Group I than group II (as measured by convexity pattern) 0.89±0.0165 vs 0.91±0.044 p<0.041). The TNF-α level were higher in Group I than Group II (p not significant).

Conclusions: Particulate matter in airways correlate to eosinophilic inflammation in asthmatic children. Air pollution may be crucial in the increasing prevalence of pediatric asthma. 
American Thoracic Society 2010 International Conference 
New Orleans, LA 
May 14-19, 2010