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2619497 
Journal Article 
Abstract 
3He and 1He MRI imaging of regional pulmonary injury in house-dust-mite allergic mice 
Nouls, J; Kaushik, SS; Potts, E; Foster, WM; Slipetz, D; Driehuys, B 
2010 
Yes 
American Journal of Respiratory and Critical Care Medicine
ISSN: 1073-449X
EISSN: 1535-4970 
181 
A6628 
English 
WOS:000208771005247 
is part of a larger document 3452678 Proceedings of the American Thoracic Society 2010 International Conference, May 14-19, 2010, New Orleans
Rationale: This study used 3He and 1H MRI for high-resolution regional assessment of ventilation and bronchial diameter before, during, and after methacholine (MCh) challenge in the house dust mite (HDM) model of airways hyperresponsiveness.

Methods: Male Balb/C mice (10 wks old, n=5) received airway instillations by oro-pharyngeal aspiration containing 100μg of D. pteronyssinus proteins suspended in 40μL PBS on days 0, 7 and 14, followed by in vivo imaging on day 15. Control mice (n=5) received only PBS. All mice underwent respiratory-gated 1H MRI at 312x312x312μm^3 resolution and then underwent a 5 minute high-resolution 3D 3He MR image (156x156x156 μm^3) before and after i.v. challenge with 125mg/kg MCh. During MCh challenge, 2D 3He MRI images were acquired with 12 second temporal and 186x186μm^2 spatial resolution for a 2 minute period.

Results: At steady-state, prior to MCh challenge, 3 out of 5 of the HDM sensitized animals showed no ventilation in their left lung lobes on 3He MRI, and 1H MRI revealed the presence of fluid in the unventilated lobes. All control animals, as expected, had homogeneous ventilation to both lung regions. During MCh challenge, 3He MRI clearly depicted constriction of the major bronchi, which was similar in magnitude in control and HDM animals. Subsequent 3D 3He MRI revealed continued bronchial airway narrowing and persistent ventilation defects in HDM treated animals versus controls.

Conclusion: The combination of high-resolution 1H and 3He MRI has the potential to provide novel approaches for studying regional pulmonary injury in small rodent models of airway obstruction. 
American Thoracic Society 2010 International Conference 
New Orleans, LA 
May 14-19, 2010