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2624214 
Journal Article 
Abstract 
Human bronchial epithelium modulates dendritic cell responses to particulate matter 
Vitte, J; Gras, D; De Senneville, L; Ferry, D; Bongrand, P; Chanez, P 
2010 
Yes 
American Journal of Respiratory and Critical Care Medicine
ISSN: 1073-449X
EISSN: 1535-4970 
181 
A3793 
English 
WOS:000208771003147 
is part of a larger document 3452678 Proceedings of the American Thoracic Society 2010 International Conference, May 14-19, 2010, New Orleans
RATIONALE: Dendritic-epithelial crosstalk plays a crucial role in sampling airway particles and locally initiating either immune response or tolerance. Combustion-derived particles are prominent air pollutants. Among these, aircraft-derived particles have gained attention in recent years, yet their health impact is unknown. Previous studies with diesel exhaust particles suggested epithelial cells exert a tight control on inhalant effects.

AIM: We therefore studied dendritic cell responses to jet engine particles with or without a bronchial epithelial barrier.

METHODS: Experimental kerosene combustion in a commercial turbofan yielded carbonaceous particles with a primary diameter of 10nm. Maturation of human monocyte-derived dendritic cells was induced by these particles, E. coli lipopopysaccharide or a combination of both. Human bronchial epithelial cells cultured in air-liquid interface established a physical barrier between apical particles and basal dendritic cells. Particle-induced modulation was studied according to phenotypic (maturation, presentation and costimulatory surface molecules), secretory (Th1/Th2 profile, chemokine production) and proliferative (induction of lymphocyte proliferation) patterns.

RESULTS: Direct contact with particles did not induce dendritic cell maturation, but modulated lipopolysaccharide-induced effects. Epithelium-mediated particle contact resulted in dendritic cell maturation even in the absence of a second stimulus. Moreover, dendritic cells displayed different maturation patterns, suggesting particle influence on subsequent immune activation and the choice of T response orientation.

CONCLUSION: Our results support the major impact of initial epithelial processing of particles before dendritic cell activation in a coculture model. 
American Thoracic Society 2010 International Conference 
New Orleans, LA 
May 14-19, 2010