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2629122 
Journal Article 
Abstract 
Epithelial-dendritic cell crosstalk: modulation of IFN type I-induced molecules by particulate matter 
Gras, D; Vitte, J; De Senneville, L; Ferry, D; Bongrand, P; Chanez, P 
2010 
Yes 
American Journal of Respiratory and Critical Care Medicine
ISSN: 1073-449X
EISSN: 1535-4970 
181 
A2845 
English 
WOS:000208771001757 
is part of a larger document 3452678 Proceedings of the American Thoracic Society 2010 International Conference, May 14-19, 2010, New Orleans
RATIONALE: The airway epithelium acts as a barrier protecting the lung from inhaled substances such as airborne pollutants. In some respiratory inflammatory disease such as asthma, these substances can either induce and/or exacerbate the disease. Situated beside airways epithelial cells and at the junction of innate and adaptative immunity, dendritic cells (DCs) play an important role in setting up how immune responses are initiated and perpetuated.

AIM: The aim of our study was to better understand how bronchial epithelial cells (BECs) could interact with DCs particularly when epithelium is exposed to environmental risk factor such as particulate matter (PM).

METHODS: For this purpose, we used a human coculture system composed of BECs differentiated in air-liquid interface and monocyte-derived dendritic cells (MDDC), maturated or not with LPS, placed on basolateral side of BECs. Particulate matters are then added on apical surface of BECs. The expression of IFN type I-induced molecules (B7-H1, B7-DC, IP-10 and CCL20), which are notably involved in T cell activation and DCs chemoattraction, was studied in BECs by RT-PCR.

RESULTS: We have shown that coculture of BECs with immature and mature MDDC could upregulate expression of some IFN type I-induced molecules, mainly B7-H1 and B7-DC. However, addition of PM in this system seems to have little effect on these expressions.

CONCLUSION: A BECs/DCs coculture system could allow mimicking the initial response of epithelium to inhaled substances and thus contribute to better understanding of the modulation of antigenic presentation and immune system activation. 
American Thoracic Society 2010 International Conference 
New Orleans, LA 
May 14-19, 2010