US EPA

2657095 

Journal Article 

Solvent-Free Synthesis of Phthalimide Under Microwave Irradiation and Modification of Talc with Synthesized Phthalimide 

Cai, YanHua 

2012 

Yes 

Asian Journal of Chemistry
ISSN: 0970-7077
EISSN: 0975-427X 

24 

2 

481-484 

WOS:000300464000002 

A simple and novel LC method has been developed for determination of isepamicin (ISP) in rat plasma, an aminoglycoside antibiotic agent. After protein precipitation and clean-up procedure to remove lipophilic contaminants, ISP is derivatized by pre-column with 9-fluorenylmethyl chloroformate for fluorescence detection. Chromatographic separations are achieved using a C18 column and mobile phase consisting of water and acetonitrile (68/32, v/v). Amikacin was used as an internal standard. The calibration curve was linear over a concentration range of 0.625-15 mu g mL(-1). The limit of quantification was 0.45 mu g mL(-1). The intra- and inter-day variabilities of ISP were both less than 5%. Both derivatives were stable for at least a week at ambient condition. This assay procedure should have useful application in therapeutic drug monitoring of ISP. The limit of detection was 0.10 mu g mL(-1). The specificity, assay linearity, low level assay linearity and assay repeatability were also investigated. The established method provides a reliable bioanalytical method to carry out isepamicin pharmacokinetics in rat plasma. 

Phthalimide; Thermal stability; Microwave irradiation; Solvent-free reaction