Health & Environmental Research Online (HERO)


Print Feedback Export to File
2663759 
Journal Article 
Rapid determination of apixaban concentration in human plasma by liquid chromatography/tandem mass spectrometry: Application to pharmacokinetic study 
Delavenne, X; Mismetti, P; Basset, T 
2013 
Yes 
Journal of Pharmaceutical and Biomedical Analysis
ISSN: 0731-7085
EISSN: 1873-264X 
78-79 
150-153 
23499913 
WOS:000317442800023 
We described the development and full validation of a rapid, high throughput sensible and accurate LC method using tandem mass spectrometry detection for determining apixaban concentration with [C-13, H-2(7)]-apixaban as internal standard in human plasma. Plasma pretreatment involved a one-step protein precipitation with methanol. The separation was performed by reverse-phase chromatography on a Luna MercuryMS C18 column (20 mm x 4 mm x 3 pm) column. The multiple reaction monitoring transitions used for quantification were m/z 460.20 -> 443.27 and 460.20 -> 198.99 for apixaban, 468.22 -> 451.30 for [C-13, H-2(7)]-apixaban in the electrospray positive ionization mode. The method was linear over the concentration range of 5-500 mu g/L. The intra- and inter-day precision values were below 14% and accuracy was from 90.0 to 105.8% at all quality control levels. Sample analysis time was less than 10 min including sample preparation. The present method was successfully applied to a pharmacokinetic study following oral administration of apixaban. (C) 2013 Elsevier B.V. All rights reserved. 
Apixaban; Direct factor Xa inhibitors; Tandem mass spectrometry; Liquid chromatography; Anticoagulants