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2725590 
Journal Article 
Simultaneous HPLC determination of multiple components in a commercial cosmetic cream 
Sottofattori, E; Anzaldi, M; Balbi, A; Tonello, G 
1998 
Yes 
Journal of Pharmaceutical and Biomedical Analysis
ISSN: 0731-7085
EISSN: 1873-264X 
BIOSIS/99/01196 
18 
1-2 
1-2 
English 
BIOSIS COPYRIGHT: BIOL ABS. A high-performance liquid chromatographic method for the simultaneous determination of magnesium ascorbyl phosphate (I), imidazolidinylurea (II), a mixture of methyl-(III), ethyl-(IV), propyl-(V), butyl-(VI) parabens dissolved in phenoxyethanol, and ascorbyl palmitate (VII), was studied by using a cyano-propyl column and a methanol gradient at 220 and 240 nm. Calibration curves were found to be linear in the 0.05-5 mg ml-1 range (compounds I, II, VII) and 0.9-160 mg ml-1 (compounds III-VI). Linear regression analysis of the data demonstrates the efficacy of the method in terms of precision and accuracy. An extraction method is developed and validated in order to apply this chromatographic method to a commercial cosmetic cream. The precision of this method, calculated as the relative standard deviation (RSD) of the recoveries (1.57-2.21%) was excellent for all compounds I-VII.