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2728459 
Journal Article 
Abstract 
The oral toxicology of dichlorodifluoromethane 
Sherman, H; Barnes, JR; Stula, EF 
1974 
1 
Toxicology and Applied Pharmacology
ISSN: 0041-008X
EISSN: 1096-0333 
29 
1 
152 
English 
is part of a larger document 3378179 Abstracts of papers for the Thirteenth Annual Meeting of the Society of Toxicology, Washington, D.C. March 10–14, 1974
Dichlorodifluoromethane has been in use since 1967 as an immersion freezing agent for food. Male and female rats have been administered dichlorodifluoromethane for 2 years by intra-gastric intubation at dose levels of 25-11 and 250-130 mg/kg, starting with offspring that had been exposed to the compound in utero. Except for a very slight decrease in the rate of body weight gain by animals that received the higher dose level, no clinical signs of toxicity were observed. The oral administration of the test compound did not affect mortality, nor did it alter any of the clinical laboratory measurements (hematology, urine analysis, liver function test). After 16 months, there was no histologic evidence of toxicity attributable to the administration of the dichlorodifluoromethane. The compound was not teratogenic to rats; it did not interfere with reproduction and lactation, and it did not alter the dominant lethal mutation index in rats. Dichlorodifluoromethane has also been administered in the diet to dogs for 2 years at levels of 300 ppm (ca. 10 mg/kg) and 3000 ppm (ca. 100 mg/kg) without any evidence of toxicity. 
Thirteenth Annual Meeting of the Society of Toxicology 
Washington, D.C. 
March 10–14, 1974