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HERO ID
2728765
Reference Type
Technical Report
Title
The FETAX (Frog Embryo Teratogenesis Assay-Xenopus) test: Evaluation of teratogenic effects induced by xenobiotics on the embryogenesis of Xenopus laevis: Modification and new applications
Author(s)
Leone, VG; Bordone, L; Cristiani, S; Sporchia, L; Vismara, C
Year
1994
Report Number
BIOSIS/97/11721
Volume
128
Issue
1
Page Numbers
3-12
Language
Italian
Abstract
BIOSIS COPYRIGHT: BIOL ABS. The Frog Embryo Teratogenesis Assay-Xenopus (FETAX) has been applied mainly in pharmacology to examine the toxicity of drugs on embryos (mortality, malformation and growth inhibition). The original FETAX methodology has been modified by us using the in vitro fertilization (ivf) and has been applied to ecotoxicological studies. One of the advantages of the modFETAX using ivf is to make the early phase of development available to the assay. In this preliminary study, we have tested chlorocresol (4-chloro-o-cresol), a soil metabolite of the pesticide MCPA (4-chloro-2-methylphenoxy acetic acid). Embryos were exposed to the chlorocresol (40, 30 mg/l of control FETAX solution) during the cleavage phase. This treatment causes a lethality in concentration-dependent manner. Lethality, evaluated at the end of the test (5 days post fertilization), was due to an high frequency of severe malformed embryos not surviving during the test.
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