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2733357 
Technical Report 
Effects of tetraconazole on sporidia of Ustilago maydis: I. A morphological study 
Carzaniga, R; Faoro, F; Farina, G; Gozzo, F 
1991 
BIOSIS/93/06372 
1 
2-3 
2-3 
English 
BIOSIS COPYRIGHT: BIOL ABS. The morphological changes in sporidia of Ustilago maydis induced by tetraconazole, depending on concentration and treatment time, were examined by Nomarski optics and fluorescence microscopy. Concentrations from 0.25 to 50 mug ml-1 affected cell division at 1.5 h exposure time. After 18 h treatment, sporidia appeared swollen, multicellular and branched. An intensive fluorescence was localized in the septa and as patches over the cell wall indicating areas of irregular chitin deposition. Far more profused changes were induced within 1.5 h when sporidia were exposed to concentrations \ 100 mug ml-1. Cells showed depressions on their surface and were no longer viable after vital staining. The morphological changes observed new information to the model of action of tetraconazole.