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2737513 
Journal Article 
Separation of lactic acid from fermented broth by solvent extraction 
Hano, T; Matsumoto, M; Uenoyama, S; Ohtake, T; Kawano, Y; Miura, S 
1992 
Yes 
Bioseparation
ISSN: 0923-179X 
BIOSIS/94/00863 
3 
5 
1992-1993 
English 
BIOSIS COPYRIGHT: BIOL ABS. The reactive extraction of lactic acid from the fermented broth was examined to develop a new recovery technique by employing various combinations of extractants and diluents. The extraction after fermentation was investigated. Since the pH of the broth after lactic acid fermentation was 2 to 2.5, the extraction based on ion-pair formation or solvation was effective. The combination of di-n-octylamine (DOA) as an extractant, hexane as a solvent, and ammonia as a stripping reagent was best from the points of the extraction-stripping performance and ease of recoveries of hexane and ammonia. The optimum conditions for in situ extraction during fermentation were examined at higher pH. In the design of in situ extraction process, the difference in the optimum pH between fermentation and extraction and the toxicity of the extractants to microbes should be taken into account. From these points, it was concluded that in situ extraction was possible by using DOA and adjusting th