Health & Environmental Research Online (HERO)


Print Feedback Export to File
2748377 
Journal Article 
Glutathione enzymes, glutathione content and t-butylhydroperoxide induced lipid peroxidation in the gill and digestive gland of the estuarine clam, Rangia cuneata 
Darby, PC; Gallagher, EP; Di Giulio, RT 
1993 
Yes 
Comparative Biochemistry and Physiology - Part C: Comparative Pharmacology and Toxicology
ISSN: 0742-8413 
BIOSIS/94/06599 
106 
3 
809-814 
English 
WOS:A1993MP73200032 
BIOSIS COPYRIGHT: BIOL ABS. 1. Reduced glutathione (GSH), glutathione reductase (GSSG-reductase) and glutathione peroxidase (GSH-peroxidase) activities were measured in the gill and digestive gland of Rangia cuneata. 2. Substantial GSH concentrations were found in both gill (820 | 80 nmole/g tissue) and digestive gland (930 | 130 nmole/g tissue). The digestive gland exhibited 2.5-fold greater GSSG-reductase activities and 0.5-fold lower GSH-peroxidase activities relative to the gill. 3. In vivo exposure to t-butyl hydroperoxide (BHP) elicited a dose-dependent increase (P and lt; 0.05) in lipid peroxidation in both tissues. Lipid peroxidation occurred earlier and to a greater extent in the digestive gland versus the gill. GSH concentrations in both tissues were unaffected by BHP exposure. 4. The study results indicate that gill and digestive gland differ in susceptibility to BHP induced oxidative damage, and the difference is accounted for by differences in tissue GSH metabolism.