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2752416 
Technical Report 
Expression of an iron-regulation hemolysin by Edwardsiella tarda 
Janda, JM; Abbott, SL 
1993 
BIOSIS/93/32234 
FED EUR MICROBIOL SOC 
FED EUR MICROBIOL SOC 
2-3 
English 
BIOSIS COPYRIGHT: BIOL ABS. The ability of Edwardsiella tarda to hemolyse red blood cells was investigated. Most E. tarda strains ( and gt; 80%) produced a hemolysin when assayed by either an agar overlay or contact-dependent hemolysis technique. This activity was cell-associated (CAH) and not released into the culture supernatant under routine conditions. When quantified, E. tarda strains significantly produced 30-40-fold higher levels of hemolytic activity against guinea pig, sheep, or rabbit erythrocytes than either E. hoshinae or E. ictaluri. When grown under iron restricted-conditions in the presence of ethylenediamine di(o-hydroxyphenylacetic acid), hemoglobin, hematin and hemin were found to stimulate growth in both liquid and agar bioassays. Hemolysin activity could be released from selected E. tarda strains when grown in L broth supplemented with EDDA; hemolytic activity was 3- to and gt; 40-fold under these conditions when compared to L broth alone. Preliminary characterization of the hemolysin