Health & Environmental Research Online (HERO)


Print Feedback Export to File
2754817 
Journal Article 
Epidermal growth factor in NMU-induced mammary tumors in rats 
Martin, G; Cricco, G; Davio, C; Engel, N; Cocca, C; Rivera, E; Bergoc, R 
1998 
Yes 
Breast Cancer Research and Treatment
ISSN: 0167-6806
EISSN: 1573-7217 
BIOSIS/98/18739 
48 
2 
175-185 
English 
BIOSIS COPYRIGHT: BIOL ABS. In this work we analyze the hypothesis that tumors induced by ip N-nitroso-N-methylurea injection express EGF-like peptides and EGF receptors which could be involved in the response to hormone manipulation. EGF receptors (EGFR) were determined in the purified membrane fraction of tumors from control and ovariectomized (OVX) animals and no significant differences were found in either maximal binding capacities (Q) or dissociation constants (Kd) between them. Neither did we observe differences between tumors that regressed (HR) or continued growing (HU) after ovariectomy. In order to test the ability of EGFR to trigger a biological response we measured the production of second messengers inositol triphosphates (IP3) and cAMP levels; we found that EGF increases IP3 production in a dose-dependent way, while cAMP levels were not affected. In addition, EGF was able to induce in vitro cell proliferation in a concentration-dependent manner when tested in primary cultures of tum