Genetics and evidence for an esterase-associated mechanism of resistance to indoxacarb in a field population of diamondback moth (Lepidoptera: Plutellidae) | Health & Environmental Research Online (HERO)

HERO ID

2770745

Reference Type

Journal Article

Title

Genetics and evidence for an esterase-associated mechanism of resistance to indoxacarb in a field population of diamondback moth (Lepidoptera: Plutellidae)

Author(s)

Sayyed, A; Wright, DJ

Year

2006

Is Peer Reviewed?

1

Journal

Pest Management Science
ISSN: 1526-498X
EISSN: 1526-4998

Publisher

John Wiley & Sons, Baffins Lane Chichester W. Sussex PO19 1UD UK, [mailto:customer@wiley.co.uk], [URL:http://www.wiley.com/]

Location

England

Volume

62

Issue

11 (Nov 2006)

Page Numbers

1045-1051

Abstract

Bioassays (at generation G2) with a newly collected field population (designated CH3) of Plutella xylostella L. from farmers' fields in the Cameron Highlands, Malaysia, indicated resistance ratios of 813-, 79-, 171-, 498- and 1285-fold for indoxacarb, fipronil, spinosad, deltamethrin and Bacillus thuringiensis toxin Cry1Ac respectively compared with a laboratory susceptible population (Lab-UK). At G2 the field-derived population was divided into two subpopulations: one was selected (G2 to G7) with indoxacarb (indoxa-SEL), while the second was left unselected (UNSEL). A significant reduction in the resistance ratio for each compound was observed in UNSEL at G8. For indoxa-SEL, bioassays at G8 found that selection with indoxacarb gave a resistance ratio of 2594 compared with Lab-UK and of 90 compared with UNSEL. The toxicity of fipronil, spinosad and deltamethrin was not significantly different in indoxa-SEL at G8 compared with G2 but was significantly greater than UNSEL at G8. The toxicity of Cry1Ac was significantly reduced in indoxa-SEL at G8 compared with G2 but was also significantly greater than UNSEL at G8. This suggests that indoxacarb selection maintained resistance to these compounds in the indoxa-SEL population. Synergist studies indicated that resistance to indoxacarb in indoxa-SEL was esterase associated. Logit regression analysis of F1 reciprocal crosses between indoxa-SEL and Lab-UK indicated that resistance to indoxacarb was inherited as an autosomal, incompletely recessive (DLC = 0.35) trait. Tests of monogenic inheritance suggested that resistance to indoxacarb was controlled by a single locus.

Keywords

Subpopulations; Plutellidae; Pest control; Toxins; fipronil; Regression analysis; Plutella xylostella; Deltamethrin; Bacillus thuringiensis; Heredity; Lepidoptera; Spinosad; Toxicity; esterase; Cry1Ac toxin