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Citation
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HERO ID
2772817
Reference Type
Technical Report
Title
Final report on the reproductive toxicity of 1-chloro-2-propanol (CAS no. 127-00-4) in Sprague-Dawley rats
Author(s)
Gulati, DK; Barnes, LH; Chapin, RE; Heindel, J
Year
1990
Report Number
DART/TER/94000340
Volume
NTIS
Issue
NTIS/PB91-158469
Page Numbers
203 pp
Language
English
Abstract
Technical grade 1-chloro-2-propanol (composed of approximately 75-76% 1-chloro-2-propanol and remaining 2-chloro-1-propanol), administered via drinking water, was tested for its effects on fertility and reproduction in CD Sprague-Dawley rats using the Continuous Breeding protocol. In a 14-day dose-finding study, 1-chloro-2-propanol at 0.2 to 0.8% adversely affected both body weight gain and water consumption. Based on these results, 0.03, 0.065, and 0.13% (w/v) in drinking water were chosen to investigate effects on fertility and reproduction. Male and female rats were continuously exposed for a 7-day precohabitation and a 16 week cohabitation period (Task 2). The chemical treatment had no effect on fertility or any of the reproductive parameters including mean number of litters, litter size, or days to delivery. Male body weights in the high dose group in Task 2 were never lower than 92% of control body weights. The female body weights varied with the gestation phase; the maximum difference was down to 86% of the control weights after 14 weeks of treatment. The water comsumption values in the treated groups were consistently lower. There clearly was no effect on fertility and reproduction in Task 2 and the cross-over mating trial (Task 3) to determine the sex affected by chemical treatment was not conducted. The F1 pups of the last litter from control and 0.13% groups were weaned for second generation studies. Live male and female pup weights were significantly lower on postnatal days 7 (female only), 14, and 21 in the treated group. Water consumption and F1 body weights were also decreased but there was no effect of 1-chloro-2-propanol exposure on F2 pup number, proportion born alive or pup weight endpoints. At necropsy, the right testis- and epididymis-to-body weight ratios were significantly increased and the incidence of abnormal sperm was significantly increased in the exposed F1 rats. Under the present experimental design, it is concluded that 1-chloro-2-propanol does not affect fertility and reproduction in F0 or F1 generation, but may adversely affect the production of morphologically normal sperm at maximally tolerated concentrations.
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