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2776071 
Journal Article 
Separation Of Phenolics (Benzoic Acids, Cinnamic Acids, Phenylacetic Acids, Quinic Acid Esters, Benzaldehydes And Acetophenones, Miscellaneous Phenolics) And Coumarins 
Vande Casteele, K; Geiger, H; Van Sumere, CF 
1983 
1 
Journal of Chromatography
ISSN: 0021-9673 
NIOSH/00142237 
258 
111-124 
A reversed phase, high performance liquid chromatography (HPLC) system was developed to separate a large series of simple phenolics and related substances. Compounds in 0.0025 to 0.025 percent solutions were applied to a prepacked column of LiChrosorb. Solvents were formic-acid/water in a 5 to 95 ratio and methanol; the methanol increased with time from 7 to 80 percent. The ultraviolet detector was set at 280 nanometers (nm). Good separation with narrow and symmetrical peaks was obtained. Retention times of 29 benzoic-acid (65850) derivatives ranged from 1.75 to 18.07 minutes. Most isomers were well separated, but different series of isomers overlapped. Eleven phenylacetic-acid (103822) derivatives had retention times of 2.42 to 15.81 minutes; phenylacetic-acid itself required detection at 260nm, but derivatives could be detected at 280nm. Retention times of 26 cinnamic-acid (621829) derivatives ranged from 6.20 to 22.22 minutes; trans and cis isomers of p-coumaric-acid (7400080) and ferulic-acid (1135246) could not be separated. Other retention times were 5.98 to 17.18 minutes for nine quinic-acid (77952) esters of benzoic-acid and cinnamic-acid, 6.04 to 25.30 minutes for 43 coumarins and furanocoumarins, 7.02 to 19.18 minutes for eight dihydrocinnamic-acids, 5.24 to 21.50 minutes for 25 benzaldehydes and acetophenones, and 1.33 to 24.99 minutes for 27 miscellaneous compounds. The authors conclude that this reversed phase HPLC system is well suited for separation of various phenolics over the whole gradient range. It eliminates the bulk of interfering highly polar materials and thereby allows further analysis of fractions, when necessary, by mass spectrometry or other techniques.