Health & Environmental Research Online (HERO)


Print Feedback Export to File
2777613 
Journal Article 
Contact allergens, but not irritants, alter receptor-mediated endocytosis by human epidermal Langerhans cells 
Rizova, H; Carayon, P; Barbier, A; Lacheretz, F; Dubertret, L; Michel, L 
1999 
Yes 
British Journal of Dermatology
ISSN: 0007-0963
EISSN: 1365-2133 
BIOSIS/99/14959 
140 
2 
200-209 
English 
BIOSIS COPYRIGHT: BIOL ABS. Allergic contact dermatitis is a T-cell-mediated inflammation, induced by contact with sensitizers and occurring through the release of epidermal cytokines and the activation of epidermal Langerhans cells (LCs). The aim of this study was to analyse early events of LC activation induced either by contact allergens or by irritants devoid of any contact allergenic properties, in order to obtain an in vitro method to discriminate between these two groups of molecules. Various contact sensitizers and o 37�C. Pre-incubation of LCs with either sensitizers or irritants increased the spontaneous internalization of HLA-DR molecules with a similar magnitude, but no clear discrimination between sensitizer and irritant effects was obtained by flow cytometry analysis. In contrast, confocal microscopy enabled discrimination between the effects of sensitizers and irritants: sensitizer-treated samples showed internalized HLA-DR molecules aggregated in large vesicles with very bright fluo