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HERO ID
2789848
Reference Type
Journal Article
Title
Tamoxifen and ATP synergistically activate Cl- release by cultured bovine pigmented ciliary epithelial cells
Author(s)
Mitchell, CH; Peterson-Yantorno, K; Coca-Prados, M; Civan, MM
Year
2000
Is Peer Reviewed?
Yes
Journal
Journal of Physiology
ISSN:
0022-3751
EISSN:
1469-7793
Volume
525
Issue
1
Page Numbers
183-193
Language
English
PMID
10811736
DOI
10.1111/j.1469-7793.2000.00183.x
Web of Science Id
WOS:000087391500018
Abstract
Purines alter aqueous humour secretion by the bilayered ciliary epithelium. Adenosine but not ATP shrinks non-pigmented ciliary epithelial (NPE) cells by activating Cl- channels. We now report effects of ATP on pigmented ciliary epithelial (PE) cells. Cultured bovine PE cells were studied volumetrically by electronic cell sorting. ATP and tamoxifen acted synergistically to shrink PE cells. Neither ATP nor tamoxifen alone had a consistent effect on cell volume. The tamoxifen, ATP-activated shrinkage required Cl- release since the response was blocked by removing Cl- and was inhibited by the Cl- channel blockers 5-nitro-2-(3-phenylpropylamino)-benzoate and 4,4'-diisothiocyano-2,2'-disulfonic acid. The modulating effect of tamoxifen could have reflected many actions of tamoxifen. Our data do not support the suggestion that tamoxifen inhibits protein kinase C (PKC) or calcium-calmodulin, or that it acts on histamine or carbachol receptors. The shrinkage produced by ATP and tamoxifen was blocked by 17beta-oestradiol, but not 17alpha-oestradiol. The cooperative interaction between tamoxifen and ATP was not mediated by an enhanced rise in [Ca2+]i. The results indicate that tamoxifen interacts synergistically with ATP to activate Cl- release by the PE cells.
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