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2793378 
Journal Article 
A simultaneous release of SOD1 with cytochrome c regulates mitochondria-dependent apoptosis 
Li, Q; Sato, EF; Zhu, X; Inoue, M 
2009 
1 
Molecular and Cellular Biochemistry
ISSN: 0300-8177
EISSN: 1573-4919 
322 
1-2 
151-159 
English 
19002561 
WOS:000263116000018 
To elucidate the significance of mitochondrial localization of Cu/Zn-SOD (SOD1), we studied the relationship between the release of mitochondrial SOD1 and apoptosis. Kinetic analysis using HL-60 cells showed that both mitochondria-dependent and mitochondria-independent pro-apoptotic drugs, such as staurosporine and actinomycin D, increased the generation of reactive oxygen species (ROS) and decreased mitochondrial membrane potential (Delta psi). ROS generation by these drugs was inhibited by Mn (III) tetrakis (5,10,15,20-benzoic acid) porphyrin (MnTBAP), a cell membrane-permeable SOD mimetic. However, MnTBAP inhibited the apoptosis induced by staurosporine but not by actinomycin D. MnTBAP failed to inhibit Delta psi decrease and release of SOD1 and cytochrome c induced by actinomycin D. Moreover, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), an inhibitor of voltage-dependent anion channel (VDAC), inhibited the release of the two proteins and apoptosis induced by staurosporine but not actinomycin D. These results suggest that ROS plays an important role in mitochondria-dependent but not mitochondria-independent apoptosis and that the release of SOD1 increases the susceptibility of mitochondria to oxidative stress, thereby enhancing a vicious cycle leading to apoptosis. 
Reactive oxygen species; Mitochondria; SOD1; Cytochrome c; Apoptosis