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2797867 
Journal Article 
Microbial structure of an anaerobic bioreactor population that continuously dechlorinates 1,2-dichloropropane 
Schlötelburg, C; Wintzingerode, C; Hauck, R; Wintzingerode, F; Hegemann, W; Göbel, UB 
2002 
Yes 
FEMS Microbiology Ecology
ISSN: 0168-6496
EISSN: 1574-6941 
Oxford University Press 
Delft 
FEMS microbiology ecology 
39 
3 
229-237 
English 
19709202 
WOS:000175663800006 
The bacterial diversity of an anaerobic 1,2-dichloropropane (DCP) dechlorinating bioreactor consortium derived from river sediment has been investigated by a combined molecular approach. By using rDNA clone libraries, denaturing gradient gel electrophoresis and quantitative real-time PCR, both Dehalococcoides ethenogenes- and Dehalobacter restrictus-like 16S rDNA sequences were found within the community. Both species are known for reductive dechlorination of tetrachloroethene. Furthermore, numerous yet-uncultured members of the Green non-sulfur bacteria occurred within the consortium. The community analyses over a period of 14 months revealed a clear population shift. D. restrictus 16S rDNA was enriched significantly and became the most abundant rDNA sequence type, suggesting that Dehalobacter spp. play a key role within the reductive dechlorination of DCP in this consortium. We propose the use of this species as an indicator to monitor the transformation process within the bioreactor. 
1,2-dichloropropane; 16S rRNA; bacterial diversity; bioreactor; reductive dechlorination; Dehalobacter restrictus