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Citation
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HERO ID
2821222
Reference Type
Journal Article
Title
Purification, identification and preliminary crystallographic studies of an allergenic protein from Solanum melongena
Author(s)
Jain, A; Salunke, DM
Year
2015
Volume
71
Issue
Pt 2
Page Numbers
221-225
Language
English
PMID
25664800
DOI
10.1107/S2053230X15000734
Abstract
Solanum melongena (eggplant), a member of the Solanaceae family, is a widely cultivated vegetable crop and is commonly used as a food throughout the world. Allergic reactions caused by members of this family are well known. However, mechanistic analyses to understand their molecular basis have not been adequately explored. In order to address this issue, the 7S vicilin protein (SM80.1) of size 45 kDa was purified from seeds of S. melongena by ammonium sulfate fractionation and size-exclusion chromatography. Significant homology of SM80.1 to an allergy-related protein from S. lycopersicum was identified through a BLAST search. Crystallization attempts with purified protein using the hanging-drop vapour-diffusion method led to hexagonal-shaped crystals. The crystals diffracted to 2.21 Å resolution and belonged to space group P6322, with unit-cell parameters a = 117.9, c = 123.5 Å.
Tags
IRIS
•
Ammonia, Oral - Problem Formulation
PubMed - 3/2015
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