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2832654 
Journal Article 
Validation of an HPLC method for the determination of imatinib mesylate in pharmaceutical dosage 
Rosasco, MA; Moyano, MA; Pizzorno, M; Segall, AI 
2005 
Yes 
Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 
28 
20 
3283-3292 
WOS:000233717700011 
This paper describes the development and validation of a new, simple, fast, and sensitive liquid chromatographic method for the determination of imatinib mesylate. Imatinib mesylate is not listed in any pharmacopoeia, and there are few methods in the literature for its quantitation in pharmaceutical dosage forms. In this work, a C 18 mu Bondapak trademark (3.9x150 mm, 5 mu m) column was used as the stationary phase, and 30 mM sodium heptane sulphonic acid in 0.01 M KH 2 PO 4 (pH 2.5):MeOH (42:58) was the mobile phase. Detection was performed on a UV detector at 237 nm. Through the evaluation of the analytical parameters, it was shown that the method is linear (r=0.9994) at concentrations ranging from 0.3 mg/mL to 0.8 mg/mL. The relative standard deviation values [RSD] for intra- and inter-day precision studies were 1.7 and 2.6. Recoveries ranged between 96.2 and 101.4. 
imatinib mesylate; HPLC; assay; stability-indicating method