US EPA

2833956 

Journal Article 

Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24 

Li, C; Yang, J; Wang, X; Wang, E; Li, B; He, R; Yuan, H 

2015 

Yes 

Bioresource Technology
ISSN: 0960-8524
EISSN: 1873-2976 

182 

18-25 

English 

25668754 

10.1016/j.biortech.2015.01.100 

WOS:000351101500003 

Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4(+)-N, NO2(-)-N and NO3(-)-N removal rates of 8.75, 7.51 and 7.73 mg L(-1)h(-1) were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4(+)-N, NO2(-)-N, NO3(-)-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4(+)-N to NO2(-)-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment. 

Phosphate accumulating bacterium; Heterotrophic nitrification; Aerobic denitrification; Pseudomonas stutzeri YG-24; Nitrogen removal