US EPA

2846203 

Journal Article 

Triphala, an Ayurvedic formulation improves the antioxidant status on TNBS induced IBD in rats 

Koppikar, SJ; Jagtap, SD; Devarshi, PP; Jangle, NM; Awad, VB; Wele, AA; Harsulkar, AM 

2014 

Yes 

European Journal of Integrative Medicine
ISSN: 1876-3820 

6 

6 

646-656 

10.1016/j.eujim.2014.08.005 

WOS:000345117500005 

Introduction: Triphala is a well-known Ayurvedic treatment for bowel problems. The aim of the study was to evaluate whether Triphala(an Ayurvedic formulation) can ameliorate excessive oxidative stress and inflammation of intestinal tract in 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced inflammatory bowel disease (IBD)in rats.



Materials and methods: Aqueous Triphala extract was assessed for Nitric oxide (NO) scavenging and anti-lipid peroxidation activities. Triphala was orally administered in three doses (330, 500 and 1000 mg/kg/b wt), where mesalamine remained as control. Activity of MDA, catalase (CAT), superoxide dismutase (SOD) and nitrate nitrite in colon tissues were estimated using plate-based assays. Gene-expression of CAT, SOD, glutathione peroxidise (GPx) and endothelial nitric oxide synthase (eNOS) were estimated using Real-Time PCR. Lastly, HE staining was used for histopathological observations.



Results: NO scavenging revealed 80.00% inhibition at 500 and 1000 mu g/ml, whereas complete inhibition of lipid peroxidation obtained at 500 and 1000 kg/ml concentrations. These activities correlated well with reduced oxidative damage (MDA 3.72 mu M/g of tissue) after Triphala treatment with significant increase in CAT, SOD and nitrate nitrite activity in colon. Gene-expression of CAT, SOD and GPx increased in dose-dependent manner, while eNOS expression increased at Triphala 500 mg/kg/b wt, demonstrating strengthening effect on innate antioxidant defence. Histopathology of colon revealed muco-protection and restoration of normal tissue architecture. Administration of Triphala at 1000 mg/kg/b wt significantly reduced colonic inflammation measured as reduced colon weight and crypt loss, reduction in infiltration by pro-inflammatory cells and focal necrotic areas in mucosa.



Conclusion: Triphala has promising potential for the treatment of IBD given its strong antioxidant effects in reducing inflammation. (C) 2014 Elsevier GmbH. All rights reserved. 

Triphala; Inflammatory bowel disease; TNBS; Antioxidant activity; ROS