Health & Environmental Research Online (HERO)


Print Feedback Export to File
2863087 
Journal Article 
Structure characterization and anti-tyrosinase mechanism of polymeric proanthocyanidins fractionated from kiwifruit pericarp 
Chai, WM; Shi, Y; Feng, HL; Xu, L; Xiang, ZH; Gao, YS; Chen, QX 
2014 
Yes 
Journal of Agricultural and Food Chemistry
ISSN: 0021-8561
EISSN: 1520-5118 
62 
27 
6382-6389 
English 
24939165 
To provide information on the structure, activity, and structure-activity relationship of kiwifruit (Actinidia chinensis) pericarp proanthocyanidins (PAs), they were separated into three fractions. These fractions were further identified by MALDI-TOF MS and HPLC-ESI-MS methods. Spectra results revealed that they are complex mixtures of B-type propelargonidins, procyanidins, procyanidins gallate, and prodelphinidins. Enzymatic activity analysis showed that these compounds strongly inhibit the activity of tyrosinase, indicating that they are reversible and mixed-type inhibitors of the enzyme. The results obtained from fluorescence quenching showed PAs inhibit the enzyme activity by interacting with substrate and enzyme. This study confirmed that the mean degree of polymerization (mDP) of PAs produces a positive effect on their anti-tyrosinase activity. In addition, the antioxidant analysis indicated that PAs possess potent antioxidant activity. These conclusions mean kiwifruit pericarp PAs may be explored as insecticides, food preservatives, and cosmetic additives.