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HERO ID
2887465
Reference Type
Journal Article
Title
Different spectra of stationary-phase mutations in early-arising versus late-arising mutants of Pseudomonas putida: Involvement of the DNA repair enzyme MutY and the stationary-phase sigma factor RpoS
Author(s)
Saumaa, S; Tover, A; Kasak, L; Kivisaar, M
Year
2002
Is Peer Reviewed?
Yes
Journal
Journal of Bacteriology
ISSN:
0021-9193
EISSN:
1098-5530
Volume
184
Issue
24
Page Numbers
6957-6965
Language
English
PMID
12446646
DOI
10.1128/JB.184.24.6957-6965.2002
Web of Science Id
WOS:000179529200023
Abstract
Stationary-phase mutations occur in populations of stressed, nongrowing, and slowly growing cells and allow mutant bacteria to overcome growth barriers. Mutational processes in starving cells are different from those occurring in growing bacteria. Here, we present evidence that changes in mutational processes also take place during starvation of bacteria. Our test system for selection of mutants based on creation of functional promoters for the transcriptional activation of the phenol degradation genes pheBA in starving Pseudomonas putida enables us to study base substitutions (C-to-A or G-to-T transversions), deletions, and insertions. We observed changes in the spectrum of promoter-creating mutations during prolonged starvation of Pseudomonas putida on phenol minimal plates. One particular C-to-A transversion was the prevailing mutation in starving cells. However, with increasing time of starvation, the importance of this mutation decreased but the percentage of other types of mutations, such as 2- to 3-bp deletions, increased. The rate of transversions was markedly elevated in the P. putida MutY-defective strain. The occurrence of 2- to 3-bp deletions required the stationary-phase sigma factor RpoS, which indicates that some mutagenic pathway is positively controlled by RpoS in P. putida.
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