Health & Environmental Research Online (HERO)


Print Feedback Export to File
2892599 
Journal Article 
Identification of oxidation product of arbutin in mushroom tyrosinase assay system 
Nihei, KI; Kubo, I 
2003 
Yes 
Bioorganic & Medicinal Chemistry Letters
ISSN: 0960-894X
EISSN: 1464-3405 
13 
14 
2409-2412 
English 
12824045 
WOS:000183947800034 
In order to elucidate whitening mechanisms of arbutin (hydroquinone-O-beta-D-glucopyranoside), its effects on mushroom tyrosinase were analyzed by spectrophotometric, polarographic, and HPLC experiments. It was found that as soon as catalytic amounts of L-DOPA become available as a cofactor, arbutin acts as a monophenol substrate. A significant enzymatic product was identified as 3,4-dihydroxyphenyl-O-beta-D-glucopyranoside by NMR and MS experiments.