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2915941 
Journal Article 
Rapid gas chromatographic method for the determination of kepone in the eel 
Meijs, AW; Ernst, GF 
1979 
1 
Journal of Chromatography
ISSN: 0021-9673 
PESTAB/79/1741 
171 
8 References 
486-489 
English 
94914 
PESTAB. Due to the contamination of the James River with the pesticide Kepone (chlordecone), and the possible contamination of eels from the river that are imported by The Netherlands, a gas chromatographic method for the determination of this compound in eels was investigated. A 20-g sample of chopped eel was macerated with anhydrous sodium sulfate in 100 ml of isopropanol: chloroform (1:1). After 16 hr the sample was centrifuged and the extract was washed by extraction with n-hexane and acetonitrile. Column chromatography was used for sample cleanup and after the residue was dissolved in 5 ml of isopropanol, a 5 mul volume of the solution was injected into the gas chromatograph. Kepone was detected in the second eluate at limits of 0.005 ppm. Extraction with isopropanol: benzene (1:1) or isopropanol: toluene (1:1) also proved successful for the analysis of Kepone residues in eel tissues. Maceration of the tissue with n-hexane or Soxhlet extraction with n-hexane gave only 25% and 50%, respectively of the amount of Kepone recovered with the present method. Little interference from other compounds was found when 48 samples of fresh, smoked or frozen eel were analyzed. Identification of Kepone in positive samples was achieved by gas chromatography mass spectrometry.