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2917945 
Journal Article 
The In Vivo Effects Of Mirex On Hepatic Lactic Dehydrogenase And Glutamic Oxaloacetic Transaminase Levels Of The Rat 
Abston, PA; Yarbrough, JD 
1974 
Yes 
Journal of Agricultural and Food Chemistry
ISSN: 0021-8561
EISSN: 1520-5118 
NIOSH/00160906 
22 
1 
66-68 
The in-vivo effects of dietary mirex (2385855) exposure on lactic-dehydrogenase (9001609) (LDH) and glutamic-oxaloacetic-transaminase (9000979) (GOT) activities were studied in rats. Sprague-Dawley-rats were fed diets containing either 10, 50, 100, or 200 parts per million (ppm) mirex for 30 days. Animals were then sacrificed, livers were removed and homogenized, and the homogenate was centrifuged to obtain the supernatant used for spectrophotometric enzyme assays. Male rats fed 10ppm mirex showed no change in LDH activity; however, rats fed both 100 and 200ppm showed about a 50 percent reduction in LDH activity. This drop appeared between days 7 and 14 of exposure and enzyme activity remained depressed for the remainder of the treatment period. Female rat liver LDH activity appeared much less sensitive to mirex exposure than male LDH activity. A drop in male liver GOT activity at 100ppm and 200ppm mirex appeared during the first 7 days of exposure and reached a 60 to 65 percent reduction at the end of 21 days. Female GOT activity was much less affected than male GOT activity. The authors conclude that tissue enzyme levels may serve as a sensitive index of chronic insecticide exposure.