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Citation
Tags
HERO ID
2963281
Reference Type
Journal Article
Title
Activation of beta 1 but not beta 3 integrin increases cell traction forces
Author(s)
Lin, GL; Cohen, DM; Desai, RA; Breckenridge, MT; Gao, L; Humphries, MJ; Chen, CS
Year
2013
Is Peer Reviewed?
Yes
Journal
FEBS Letters
ISSN:
0014-5793
EISSN:
1873-3468
Volume
587
Issue
6
Page Numbers
763-769
Language
English
PMID
23395612
DOI
10.1016/j.febslet.2013.01.068
Web of Science Id
WOS:000315947400035
Abstract
Cell-generated traction forces induce integrin activation, leading to focal adhesion growth and cell spreading. It remains unknown, however, whether integrin activation feeds back to impact the generation of cytoskeletal tension. Here, we used elastomeric micropost arrays to measure cellular traction forces in wildtype and integrin-null cells. We report that activation of β1 but not β3 integrin, by either increasing density of immobilized fibronectin or treating with manganese, elicited fibroblast spreading and cytoskeletal tension. Furthermore, this force generation required Rho kinase and myosin activity. These findings suggest that integrin activation and cell traction forces comprise a bi-directional signaling unit of cell adhesion.
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