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2967667 
Journal Article 
Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae 
Wu, HJ; Seib, KL; Srikhanta, YN; Edwards, J; Kidd, SP; Maguire, TL; Hamilton, A; Pan, KT; Hsiao, HH; Yao, CW; Grimmond, SM; Apicella, MA; Mcewan, AG; Wang, AH; Jennings, MP 
2010 
Yes 
Journal of Proteomics
ISSN: 1874-3919 
73 
5 
899-916 
English 
20004262 
WOS:000276167900005 
Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e.g., pilin, a key adhesin), oxidative stress defence (e.g., superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division. Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defence. A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress. Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance.