US EPA

2972564 

Technical Report 

Microlocalization and Quantitation of Risk Associated Elements in Gleason Graded Prostate Tissue 

Eckhert, CD 

2005 

NTIS/02937135 

GRA and I 

GRA and I 

There is growing evidence that the elements boron, selenium and zinc reduce PCa risk whereas calcium and cadmium increase risk (1-14). The objective of this proposal is to determine if these elements differ in concentration and location between normal and tumor tissue. During the first year of the project pathologically graded human prostate tissue was analyzed to determine the concentrations of boron, calcium, cadmium, selenium and zinc. There was no statistical association between gross tissue concentrations and Gleason score. The major goals for the second year were to receive training on use of the NanoSIMS microscopy at Lawrence Livermore National Laboratory (LLNL), develop sample preparation procedures for the analysis of tissue on the instrument, and begin analysis. These goals were accomplished and early analysis is showing that each element has a unique cellular distribution pattern. During the third project year we will concentrate on characterizing the compartmentalization of the elements both within cells and within prostate tissue of different Gleason grades. It is expected that elemental location, rather than concentration, will provide insight into how the elements under study are able to modify PCa risk.